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Flashcards in this deck (56)
  • What does it mean that viruses are 'obligate intracellular parasites'?

    They must enter a host cell and take over its molecular processes to produce more viral particles.

    virology definition
  • Why do many viruses have a narrow host range?

    Because a virus must recognize a specific structure on the cell surface to enter the host cell.

    host-range entry
  • Give the size range examples for the largest and smallest viruses mentioned.

    • Largest: Pox viruses ~0.3 micron
    • Smallest: Polio virus ~30 nm
    size measurement
  • Why were early viruses called 'filterable agents'?

    Because they caused disease but passed through the filters of the day and could not be retained by those filters.

    history discovery
  • Compare the genome sizes of Smallpox and Polio as given.

    • Smallpox: ~300 kb DNA
    • Polio: ~7 kb nucleic acid
    genome sizes
  • How do viruses manage with very small genomes?

    Viral genes overlap significantly and many proteins have multiple functional sites with different enzymatic activities.

    genome organization
  • What is a capsid and what is a nucleocapsid?

    • Capsid: protein shell that encloses viral nucleic acid
    • Nucleocapsid: nucleic acid contained inside the capsid
    structure capsid
  • What nucleic acid forms are found in viruses according to the text?

    Nucleic acid can be single-stranded or double-stranded; single-stranded RNA can be plus-strand (resembles mRNA) or minus-strand (complementary to mRNA).

    genome classification
  • How do enveloped viruses acquire their envelope?

    They acquire it from budding out of the host cell membrane, taking a piece of membrane as an envelope.

    envelope entry
  • What advantage does an envelope give a virus regarding the immune system?

    Being clothed in a piece of host membrane can help the virus fool the immune system.

    immune-evasion envelope
  • Contrast enveloped and naked (non-enveloped) viruses in infectivity and environmental stability.

    Enveloped viruses are very infectious but not environmentally tough; naked viruses are environmentally tough but not especially infectious.

    stability infectivity
  • What is noted about the fidelity of virus replication?

    Virus replication is inherently sloppy, producing many incomplete or badly made particles alongside functional ones.

    replication fidelity
  • What is described as the best defense against viral infection?

    Vaccination is described as the best defense against viral infection.

    prevention vaccines
  • What is a common limitation of most recently developed antiviral pharmaceuticals?

    They are specific to a small group of viruses and not broad-spectrum.

    antiviral pharmacology
  • When are antisera (passive immunity) used for viral infections?

    For certain rare but dangerous viral infections.

    immunology therapy
  • What is the usual approach to treatment for many viral infections like influenza?

    Supportive therapy.

    clinical therapy
  • What classification system is generally used to describe viruses by how their nucleic acid produces mRNA?

    The Baltimore system.

    classification virology
  • Which single-stranded DNA virus causes Fifth Disease in humans?

    Parvovirus B19.

    parvovirus pathogen
  • What is a characteristic clinical sign of Fifth Disease in children?

    A 'slapped cheek' rash.

    clinical pediatrics
  • What happens early after Parvovirus B19 infection at the molecular level?

    The single strand is copied into double-stranded DNA using a unique polymerase.

    replication molecular
  • What dog disease is mentioned as very dangerous and caused by a parvovirus?

    Canine parvovirus is very dangerous for dogs.

    parvovirus veterinary
  • Name one feature of Smallpox (Variola) mentioned in the text.

    It is highly contagious and enveloped.

    poxvirus smallpox
  • Why did Edward Jenner's cowpox inoculation protect against Smallpox?

    Because milkmaids who got cowpox did not get Smallpox and Smallpox had only one serotype.

    vaccination history
  • What is the current status of Smallpox as a pathogen according to the text?

    It has been eliminated as a pathogen and exists only in research laboratories.

    publichealth eradication
  • Give one example of a mild poxvirus illness in children mentioned in the text.

    Molluscum Contagiosum, which produces small persistent papules.

    poxvirus clinical
  • What outbreak statistics for monkeypox are given in the text?

    About 30,000 cases in the U.S. and 20 deaths.

    monkeypox epidemiology
  • List two shared characteristics of the Herpesviruses mentioned in the text.

    • They are enveloped
    • They are spread by close contact
    herpesvirus characteristics
  • Name two additional shared features of Herpesviruses from the text.

    • Envelope derived from the nuclear membrane
    • Most have a latent form
    herpesvirus virology
  • How many herpesviruses does the text state exist, and how many will be examined?

    There are eight herpesviruses and five will be examined.

    herpesvirus overview
  • What is Human Herpesvirus 1 (HHV1) commonly called?

    Herpes Simplex type 1 (oral herpes)

    herpes virology
  • Where does HHV1 persist in the body during latency?

    In sensory neurons

    herpes latency
  • What antiviral is mentioned as helpful for HHV1?

    Topical acyclovir

    treatment herpes
  • What is the typical disease caused by Human Herpesvirus 2 (HHV2)?

    Genital herpes

    herpes std
  • How can oral and genital herpes be definitively distinguished?

    By molecular analysis

    diagnosis herpes
  • What disease does Varicella Zoster Virus (VZV) cause initially and what can it re-emerge as?

    • Initially causes chicken pox
    • Can re-emerge as shingles
    vzv disease
  • How is VZV primarily spread during primary infection?

    By respiratory droplets

    transmission vzv
  • What is the target cell type for Epstein-Barr Virus (HHV4)?

    B lymphocytes

    ebv immunology
  • Which cancers are associated with HHV4 (Epstein-Barr Virus)?

    • Burkitt's lymphoma
    • Hodgkin's disease
    ebv oncology
  • In which cells does Cytomegalovirus (HHV5) remain latent?

    Monocytes and lymphocytes

    cmv latency
  • Name major neonatal complications associated with congenital CMV (HHV5).

    • Microcephaly
    • Hepatosplenomegaly
    • Deafness
    • Mental retardation
    cmv neonates
  • Which HHV is called 'sixth disease' and where does it go latent?

    HHV6 (roseola infantum) goes latent in T cells

    hhv6 latency
  • Which herpesvirus is associated with Kaposi's sarcoma and in which cells is it latent?

    HHV8 is associated with Kaposi's sarcoma and is latent in B cells

    hhv8 oncology
  • How is Hepatitis B virus (HBV) transmitted?

    • Sexually (STD)
    • Through needle sticks
    hbv transmission
  • What unusual feature does the HBV genome have that affects replication?

    It is not completely double-stranded and has ragged ends that must be completed using reverse transcriptase

    hbv replication
  • What effect does co-infection with Hepatitis D Virus have on HBV disease severity?

    Co-infection with Hepatitis D Virus makes the disease far worse and increases liver cancer risk in severe cases

    hbv co-infection
  • Is there a vaccine for Hepatitis B virus (HBV)?

    Yes, a vaccine is available and has greatly reduced infections

    hbv prevention
  • What illnesses can adenovirus cause?

    • Many respiratory infections
    • Occasionally pneumonia
    • Conjunctivitis (pinkeye)
    • Gastroenteritis depending on serotype
    adenovirus disease
  • Where can adenovirus establish latency and when is reemergence usually a problem?

    It can go latent and reemerge later, usually a problem only in the immunocompromised

    adenovirus latency
  • What are papillomaviruses (HPV) commonly known to cause?

    Warts (often harmless but sometimes unsightly or irritating)

    papillomavirus hpv
  • How are genital wart-causing human papillomavirus (HPV) serotypes transmitted?

    They are transmitted sexually.

    hpv transmission
  • Why can certain HPV serotypes potentially cause cancer?

    Because they can incorporate into host chromosomes, creating potential to cause cancer.

    hpv oncology
  • Which HPV serotypes are notorious for causing cervical cancer?

    • HPV 16
    • HPV 18
    hpv cervicalcancer
  • Which HPV serotypes cause laryngeal and anogenital warts mentioned in the text?

    • HPV 6
    • HPV 11
    hpv warts
  • Which HPV vaccine replaced Cervarix and how many serotypes does it protect against?

    Gardasil-9 replaced Cervarix and protects against 9 serotypes.

    hpv vaccine
  • Which viruses are included in the Polyoma virus group that are highlighted as unusual?

    • BK virus
    • JC virus
    polyomavirus virology
  • Who should be concerned about Polyoma viruses like BK and JC?

    Only immunocompromised individuals should be concerned, as these viruses rarely cause disease.

    polyomavirus immunology
Study Notes

Overview

  • Viruses are obligate intracellular parasites: they must enter host cells to replicate.
  • They typically have a narrow host range because entry requires recognition of specific cell surface structures.
  • Virus study advanced with bacteriophage research, tissue culture, electron microscopy, and sequencing.

Key properties (quick)

  • Size: range from ~30 nm (poliovirus) to ~0.3 micron (poxviruses). Small size required new detection methods historically.
  • Genome: very compact; genes often overlap and viral proteins may be multifunctional.
  • Capsid and envelope: protein capsid encloses nucleic acid; some viruses have a host-derived lipid envelope.
  • Environmental stability vs infectivity: enveloped viruses are generally more infectious but fragile; naked viruses are tougher in the environment but often less infectious.
  • High production error rate: many defective particles are produced, but enough functional virions sustain infection.

Structure and replication basics

  • Viral particle = capsid (protein shell) + genome (DNA or RNA); when membrane present, the whole is an enveloped virion.
  • Genome types: ssDNA, dsDNA, ssRNA (+ or -), dsRNA, or reverse-transcribing (e.g., hepadna/retroviruses).
  • Single-stranded RNA + strand resembles mRNA; - strand is complementary and must be transcribed to + for translation.
  • Capsid proteins are few and usually self-assemble from one or two gene products due to limited genome capacity.

Clinical control: vaccines and antivirals

  • Vaccination is the best preventive measure for many viral diseases (historical example: smallpox eradication).
  • Antiviral drugs exist but are often virus-specific; some reverse transcriptase inhibitors can work across viruses with similar enzymes (e.g., HBV and HIV drugs overlap).
  • Passive immunotherapy (antisera/gamma globulin) is reserved for certain exposures or vulnerable patients.

Classification approach used here

  • Presentation organized by nucleic acid type, starting with DNA viruses then RNA viruses (note: Baltimore classification groups viruses by how mRNA is produced).

DNA viruses — essentials

Single-stranded DNA

  • Parvovirus B19
  • Causes erythema infectiosum (fifth disease) in children, often mild with a 'slapped-cheek' rash.
  • Uses host machinery to convert ssDNA to dsDNA early after infection.
  • Related parvoviruses can be important in animals (canine parvovirus, feline parvoviruses).

Double-stranded DNA

  • Poxviruses
  • Large dsDNA viruses (largest human viruses); variola (smallpox) is historically significant.
  • Enveloped and highly contagious by close contact; smallpox had one serotype and was eradicated by vaccination.
  • Examples: smallpox, cowpox, molluscum contagiosum, monkeypox (recent outbreaks).

  • Herpesviruses (HHV family) — enveloped, latent, widespread seropositivity; derived from nuclear membrane

  • HHV1 (HSV-1): oral herpes; latency in sensory neurons; topical acyclovir helps.
  • HHV2 (HSV-2): genital herpes; sexually transmitted; can be asymptomatic between outbreaks.
  • Varicella zoster virus (VZV): causes chickenpox; latent in neurons and can reactivate as shingles; spread by respiratory droplets.
  • HHV4 (Epstein-Barr virus): infects B cells, causes mononucleosis; associated with Burkitt lymphoma and Hodgkin disease.
  • HHV5 (Cytomegalovirus): often mild in adults but can cause severe congenital disease (microcephaly, deafness).
  • HHV6/HHV7: common childhood infections (roseola), latency in T cells.
  • HHV8: associated with Kaposi sarcoma, mainly in immunocompromised patients.
  • Clinical notes: all can establish latency, reactivate, and cause more severe disease in immunocompromised hosts.

  • Hepadnavirus (Hepatitis B virus, HBV)

  • Partially double-stranded DNA with gapped/ragged ends; replication involves a reverse transcriptase step.
  • Transmitted sexually and via blood/needlesticks; co-infection with Hepatitis D worsens disease and increases cancer risk.
  • Vaccine available and effective; some HIV RT inhibitors are useful against HBV due to similar enzymatic steps.

  • Adenovirus

  • Causes a range of usually mild respiratory illnesses, conjunctivitis, and gastroenteritis depending on serotype.
  • Can establish latency; severe disease mainly in immunocompromised.
  • Live vaccines for certain serotypes have existed but are not widely used in the general population.

  • Papillomavirus (HPV)

  • Causes warts (cutaneous and genital); many serotypes with differing disease risks.
  • Certain high-risk types (notably 16 and 18) integrate into host genome and are strongly linked to cervical cancer.
  • Vaccines (e.g., Gardasil-9) protect against multiple oncogenic and wart-causing serotypes.

  • Polyomaviruses (BK, JC, etc.)

  • Usually asymptomatic in healthy people; can cause serious disease in immunosuppressed patients (BK—kidney, JC—progressive multifocal leukoencephalopathy).

Practical points for study

  • Remember the association: enveloped = fragile but often spread by intimate contact or respiratory routes; naked = environmentally stable, often fecal-oral or fomites.
  • Latency is a key concept for herpesviruses; reactivation causes clinical recurrence and transmission risk.
  • Vaccine examples: smallpox (eradicated), HBV (reduces chronic carriers and liver cancer risk), HPV (prevents cervical and other cancers).
  • Antivirals: many target viral-specific enzymes (polymerases, reverse transcriptases, proteases) and are usually narrow-spectrum.

Rapid comparison table (essentials)

  • Poxviruses: large dsDNA, enveloped, vaccines historically crucial
  • Herpesviruses: dsDNA, enveloped, latent in neurons or leukocytes
  • HBV (hepadnavirus): partially dsDNA, reverse transcription step, vaccine available
  • Adenovirus: dsDNA, non-enveloped, respiratory/conjunctival/gastrointestinal disease
  • Papillomavirus: dsDNA, non-enveloped, integrates into host genome, some types oncogenic
  • Polyomavirus: dsDNA, usually subclinical, important in immunosuppression

Summary tips

  • Focus on genome type, envelope presence, tropism (which cells they infect), latency, transmission route, and available prevention (vaccines) when learning viruses.
  • Grouping by nucleic acid helps predict replication strategy and antiviral targets.