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What is the focus of Molecular and Cell Biology?
Study of cells, their structures, functions, and interactions.
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What university offers BIOL 102?
Queen's University
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Which week is referred to in the lecture title?
Week 2
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What is the logo of Queen's University used for?
Visual representation of the university.
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What is the due date for DSMs 6-7?
Due Saturday Sep 20 at 11:59 PM. Grace period ends Tuesday Sep 23 at 11:59 PM.
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When does Lecture Quiz 1 open?
Opens Saturday Sep 13 at 12:01 AM.
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What is the due date for Lecture Quiz 1?
Due Monday Sep 15 at 11:59 PM. Grace period ends Thursday Sep 18 at 11:59 PM.
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When does Lab 1A - Background Research quiz open?
Opens Monday Sep 8 at 12:01 AM.
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What is the due date for Lab 1A quiz?
Due Thursday Sep 11 at 11:59 PM. Grace period ends Sunday Sep 14 at 11:59 PM.
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When is the due date for Stuff You Should Know activity?
Due Thursday Sep 25 at 11:59 PM. Grace period ends Sunday Sep 28 at 11:59 PM.
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When do in-person labs start?
In-person labs don’t start until Week 3.
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What does the schedule include?
It includes lecture material, quizzes, and assignment deadlines. 
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What is light microscopy based on?
Passing a beam of light through a specimen
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What does electron microscopy use?
A beam of electrons over or through a specimen
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Define resolution in microscopy.
Minimum distance two points can be separated and still distinguishable
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What is the formula for magnification?
Ratio of an object's image size to its real size
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What does contrast refer to?
Difference in brightness between light and dark areas
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What does this diagram illustrate?
Scales of biological entities and microscopy ranges 
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What is the process of phagocytosis? 
Macrophage digesting bacteria.
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What structures are shown in the microscopic view? 
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Where in the cell would a culture of rapidly growing animal cells have the greatest concentration of radioactivity when adding non-toxic radioactive dTTP?
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Where is the DNA found in eukaryotic cells?
In the nucleus
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Eukaryotic cells are subdivided into which two types?
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What is the interior substance of a cell called?
The cytoplasm
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What jelly-like substance is found in the cytoplasm?
The cytosol
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What is present in plant cells but absent in animal cells?
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What is unique about the vacuole in plant cells?
One large central vacuole
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Which structure is common to all animal cells?
Presence of centrioles
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What organelle is responsible for energy production in both cell types?
Mitochondrion
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Which organelle performs photosynthesis in plant cells?
Chloroplast
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In which type of cell are lysosomes mostly found?
Animal cells
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Which structure is absent in most plant cells?
Cilia
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What structure supports the plant cell and is made of cellulose?
Cell wall
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What diagram shows the organelles in a typical animal cell?

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What diagram shows the organelles in a typical plant cell?

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What do most plant cells look like?
They resemble the standard textbook image of cells.
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What is a distinguishing feature of plant cells?
They have visible cell walls.
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What can be seen clearly in most plant cells?
Visible nuclei within the cells.
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What is shown in the microscopic image of onion cells?
Rows of onion cells with distinct cell walls and nuclei. 
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What is the shape of epidermal pavement cells?
These cells have an interlocking, irregular shape.
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What organelles are labeled in the epidermal pavement cell diagram?
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How do epidermal pavement cells contribute to plants?
They provide protection and add strength & stability.
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Describe the appearance of green fluorescent plant epidermal cells.
They have a characteristic jigsaw puzzle shape.
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What are ribosomes composed of?
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What is the primary role of ribosomes?
Protein synthesis
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Where are free ribosomes located?
Cytosol
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What are bound ribosomes associated with?
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What does a ribosome consist of?
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What is the structure of a ribosome?
3D model with complex structure.
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What are the components of the endomembrane system?
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How are the components of the endomembrane system connected?
Through direct physical continuity or the transfer of vesicles.
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What does the diagram of the endomembrane system include?
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What does the nucleus contain?
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What is the function of the nucleus?
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What is the nuclear envelope?
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What provides the stability to the nuclear envelope?
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What occurs in the nucleolus?
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What accompanies the structure of the nucleus in diagrams?

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What does the label in the nuclear envelope diagram indicate?

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What is the main function of the Smooth ER?
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What does the Rough ER synthesize proteins for?
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What processes is the Rough ER involved in?
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What does the diagram illustrate?
The structure of the rough and smooth endoplasmic reticulum
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What is the function of the Golgi apparatus?
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What does the Golgi apparatus alter?
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What does the Golgi apparatus synthesize?
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What are the two faces of the Golgi apparatus?
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What are lysosomes?
Membranous sacs with an acidic interior containing hydrolytic enzymes for digestion.
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What is the function of hydrolytic enzymes in lysosomes?
They are used for the hydrolysis of macromolecules.
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What process involves the fusion of a food vacuole with a lysosome?
This process is called phagocytosis.
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What is autophagy?
A process in which a vesicle containing cellular debris fuses with a lysosome for digestion.
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What are vacuoles?
Large vesicles with an internal solution differing in composition from the cytoplasm.
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What is the function of the central vacuole?
Important for maintaining cell structure.
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What does the central vacuole contain?
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Where is the central vacuole found?
In plant cells.
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What is shown in the electron micrograph related to vacuoles?
The structure of the central vacuole within a plant cell.
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What are the primary sites of cellular respiration?
Mitochondria produce ATP from fuels and oxygen.
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What are the main functions of chloroplasts?
Sites of photosynthesis, producing sugars from carbon dioxide, water, and light.
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What genetic material do mitochondria and chloroplasts contain?
Both contain their own DNA and ribosomes.
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How do the ribosomes of mitochondria and chloroplasts compare?
They are more related to bacteria than to eukaryotic versions.
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What does the Endosymbiont Theory describe?
The evolutionary origins of mitochondria and chloroplasts.
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What are the components involved in the Endosymbiont Theory?
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What is illustrated in the diagram of the Endosymbiont Theory?
An ancestral eukaryotic cell engulfing a nonphotosynthetic prokaryote to become a mitochondrion, and a photosynthetic prokaryote to become a chloroplast. 
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Do alternative theories to the Endosymbiont Theory exist?
Yes, alternate theories exist.
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A mutation affecting polysaccharide modifications to proteins would likely disrupt which cellular structures?
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What are the possible structures affected by the mutation?
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What is the cytoskeleton?
A dynamic network of fibers extending throughout the cytoplasm.
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What are the three main components of the cytoskeleton?
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What does the cytoskeleton provide for the cell?
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What role does the cytoskeleton play in cell motility?
Involved in the movement of the cell location and the movement of cell parts.
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What colors represent the components of the cytoskeleton in the micrograph?
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What are the main components of the cytoskeleton?
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What is the structure of Microtubules?
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What are the protein subunits of Microtubules?
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What is the main function of Microtubules?
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What is the structure of Microfilaments?
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What are the main functions of Microfilaments?
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What is the structure of Intermediate Filaments?
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What is the main function of Intermediate Filaments?
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What does the microtubule diagram represent?
Diagram of a column of tubulin dimers, structural unit of microtubules.

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What does the microfilament diagram illustrate?
Diagram of an actin filament, composed of two intertwined strands of actin subunits.

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What does the intermediate filament diagram show?
Diagram of keratin proteins coiled together to form a fibrous subunit of intermediate filaments.

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What structure is involved in the cytoskeleton?
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What are microtubules made of?
Tubulin Polymers
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What are microfilaments mainly composed of?
Actin Filaments
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What do you call the fibers that help maintain cell shape?
Intermediate Filaments
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What are plasmodesmata?
Channels connecting plant cells, allowing transfer of compounds.
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What do gap junctions do?
Allow passage of ions/molecules between animal cells.
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What is the function of tight junctions?
Create seals between cells to prevent extracellular fluid movement.
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What do desmosomes do?
Fasten cells together using intermediate filaments.
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What structure is labeled in this diagram? 
Plasmodesmata, allowing cell-to-cell communication.
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What is the purpose of the tight junctions indicated in this diagram? 
Prevent intracellular fluid movement.
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Which organs would dysfunction due to abnormal microtubules?
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Which choice includes areas of contraction affected by microtubule dysfunction?
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What tissues have projections that might be affected by abnormal microtubules?
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Which ducts may experience dysfunction due to abnormal microtubules?
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Which cells showing movement may be affected by abnormal microtubules?
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What type of cells exhibit amoeboid movement that may be impacted?
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What is the primary function of hepatocytes?
Drug detoxification, lipid synthesis/export, protein secretion.
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What sub-cellular structures do hepatocytes have?
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How many mitochondria do hepatocytes contain?
1000-2000 mitochondria per cell.
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What happens to hepatocytes under chronic exposure to toxins?
Proliferation of smooth ER and increased drug tolerance.
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What does the smooth ER in hepatocytes assist with?
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What does the rough ER in hepatocytes mainly function for?
Protein secretion.
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Show an image of hepatocyte ER structures.

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Do erythrocytes (red blood cells) have a nucleus?
No, they do not have a nucleus in mammals.
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What type of energy production do erythrocytes rely on?
ATP is produced from fermentation, as they lack mitochondria.
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What cellular structures are absent in erythrocytes?
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Why can't erythrocytes be targeted by viruses?
They lack DNA and RNA, preventing protein synthesis.
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What is important for erythrocyte deformability?
The actin cytoskeleton and fluid cell membrane are crucial.
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How does the blood type get determined?
Blood type is determined by the presence of glycoproteins.
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What are the types of blood glycoproteins?
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What is shown in this electron micrograph?
An erythrocyte (red blood cell). 
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What is illustrated in the diagram of blood cells?
Different types of blood cells: O, A, B, AB. 
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What are keratinocytes?
Cells that form the epidermis of the skin.
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What happens to keratinocytes as they mature?
They lose their nucleus and other organelles.
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What provides rigidity to keratinocytes?
An extensive network of intermediate filaments (keratins).
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What role do desmosomes play in keratinocytes?
They provide adhesion and help in wound healing.
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Do keratinocytes continue to divide?
They permanently withdraw from the cell cycle.
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What is shown in the skin layers diagram?
The surface, upper keratinocytes, melanocytes, and basal keratinocytes.
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What is the structure composition of skeletal muscle cells?
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What is the basic contractile unit of muscle?
The sarcomere
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What connects the sarcomere to other organelles?
Intermediate filaments
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What is the role of sarcoplasmic reticulum in muscle cells?
Stores calcium, important for contraction
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What condition is related to calcium leak in muscle cells?
Rigor mortis
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What do skeletal muscle cells require to produce energy?
Lots of ATP from mitochondria
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What is the role of actin and myosin?
Required for contraction of muscle cells
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What do you observe in light micrograph of muscle cells?
Striated appearance
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What is the focus of Molecular and Cell Biology?
Study of cells, their structures, functions, and interactions.
What university offers BIOL 102?
Queen's University
Which week is referred to in the lecture title?
Week 2
What is the logo of Queen's University used for?
Visual representation of the university.
What is the due date for DSMs 6-7?
Due Saturday Sep 20 at 11:59 PM. Grace period ends Tuesday Sep 23 at 11:59 PM.
When does Lecture Quiz 1 open?
Opens Saturday Sep 13 at 12:01 AM.
What is the due date for Lecture Quiz 1?
Due Monday Sep 15 at 11:59 PM. Grace period ends Thursday Sep 18 at 11:59 PM.
When does Lab 1A - Background Research quiz open?
Opens Monday Sep 8 at 12:01 AM.
What is the due date for Lab 1A quiz?
Due Thursday Sep 11 at 11:59 PM. Grace period ends Sunday Sep 14 at 11:59 PM.
When is the due date for Stuff You Should Know activity?
Due Thursday Sep 25 at 11:59 PM. Grace period ends Sunday Sep 28 at 11:59 PM.
When do in-person labs start?
In-person labs don’t start until Week 3.
What does the schedule include?
It includes lecture material, quizzes, and assignment deadlines. 
What is light microscopy based on?
Passing a beam of light through a specimen
What does electron microscopy use?
A beam of electrons over or through a specimen
Define resolution in microscopy.
Minimum distance two points can be separated and still distinguishable
What is the formula for magnification?
Ratio of an object's image size to its real size
What does contrast refer to?
Difference in brightness between light and dark areas
What does this diagram illustrate?
Scales of biological entities and microscopy ranges 
What is the process of phagocytosis? 
Macrophage digesting bacteria.
What structures are shown in the microscopic view? 
Where in the cell would a culture of rapidly growing animal cells have the greatest concentration of radioactivity when adding non-toxic radioactive dTTP?
Where is the DNA found in eukaryotic cells?
In the nucleus
Eukaryotic cells are subdivided into which two types?
What is the interior substance of a cell called?
The cytoplasm
What jelly-like substance is found in the cytoplasm?
The cytosol
What is present in plant cells but absent in animal cells?
What is unique about the vacuole in plant cells?
One large central vacuole
Which structure is common to all animal cells?
Presence of centrioles
What organelle is responsible for energy production in both cell types?
Mitochondrion
Which organelle performs photosynthesis in plant cells?
Chloroplast
In which type of cell are lysosomes mostly found?
Animal cells
Which structure is absent in most plant cells?
Cilia
What structure supports the plant cell and is made of cellulose?
Cell wall
What diagram shows the organelles in a typical animal cell?

What diagram shows the organelles in a typical plant cell?

What do most plant cells look like?
They resemble the standard textbook image of cells.
What is a distinguishing feature of plant cells?
They have visible cell walls.
What can be seen clearly in most plant cells?
Visible nuclei within the cells.
What is shown in the microscopic image of onion cells?
Rows of onion cells with distinct cell walls and nuclei. 
What is the shape of epidermal pavement cells?
These cells have an interlocking, irregular shape.
What organelles are labeled in the epidermal pavement cell diagram?
How do epidermal pavement cells contribute to plants?
They provide protection and add strength & stability.
Describe the appearance of green fluorescent plant epidermal cells.
They have a characteristic jigsaw puzzle shape.
What are ribosomes composed of?
What is the primary role of ribosomes?
Protein synthesis
Where are free ribosomes located?
Cytosol
What are bound ribosomes associated with?
What does a ribosome consist of?
What is the structure of a ribosome?
3D model with complex structure.
What are the components of the endomembrane system?
How are the components of the endomembrane system connected?
Through direct physical continuity or the transfer of vesicles.
What does the diagram of the endomembrane system include?
What does the nucleus contain?
What is the function of the nucleus?
What is the nuclear envelope?
What provides the stability to the nuclear envelope?
What occurs in the nucleolus?
What accompanies the structure of the nucleus in diagrams?

What does the label in the nuclear envelope diagram indicate?

What is the main function of the Smooth ER?
What does the Rough ER synthesize proteins for?
What processes is the Rough ER involved in?
What does the diagram illustrate?
The structure of the rough and smooth endoplasmic reticulum
What is the function of the Golgi apparatus?
What does the Golgi apparatus alter?
What does the Golgi apparatus synthesize?
What are the two faces of the Golgi apparatus?
What are lysosomes?
Membranous sacs with an acidic interior containing hydrolytic enzymes for digestion.
What is the function of hydrolytic enzymes in lysosomes?
They are used for the hydrolysis of macromolecules.
What process involves the fusion of a food vacuole with a lysosome?
This process is called phagocytosis.
What is autophagy?
A process in which a vesicle containing cellular debris fuses with a lysosome for digestion.
What are vacuoles?
Large vesicles with an internal solution differing in composition from the cytoplasm.
What is the function of the central vacuole?
Important for maintaining cell structure.
What does the central vacuole contain?
Where is the central vacuole found?
In plant cells.
What is shown in the electron micrograph related to vacuoles?
The structure of the central vacuole within a plant cell.
What are the primary sites of cellular respiration?
Mitochondria produce ATP from fuels and oxygen.
What are the main functions of chloroplasts?
Sites of photosynthesis, producing sugars from carbon dioxide, water, and light.
What genetic material do mitochondria and chloroplasts contain?
Both contain their own DNA and ribosomes.
How do the ribosomes of mitochondria and chloroplasts compare?
They are more related to bacteria than to eukaryotic versions.
What does the Endosymbiont Theory describe?
The evolutionary origins of mitochondria and chloroplasts.
What are the components involved in the Endosymbiont Theory?
What is illustrated in the diagram of the Endosymbiont Theory?
An ancestral eukaryotic cell engulfing a nonphotosynthetic prokaryote to become a mitochondrion, and a photosynthetic prokaryote to become a chloroplast. 
Do alternative theories to the Endosymbiont Theory exist?
Yes, alternate theories exist.
A mutation affecting polysaccharide modifications to proteins would likely disrupt which cellular structures?
What are the possible structures affected by the mutation?
What is the cytoskeleton?
A dynamic network of fibers extending throughout the cytoplasm.
What are the three main components of the cytoskeleton?
What does the cytoskeleton provide for the cell?
What role does the cytoskeleton play in cell motility?
Involved in the movement of the cell location and the movement of cell parts.
What colors represent the components of the cytoskeleton in the micrograph?
What are the main components of the cytoskeleton?
What is the structure of Microtubules?
What are the protein subunits of Microtubules?
What is the main function of Microtubules?
What is the structure of Microfilaments?
What are the main functions of Microfilaments?
What is the structure of Intermediate Filaments?
What is the main function of Intermediate Filaments?
What does the microtubule diagram represent?
Diagram of a column of tubulin dimers, structural unit of microtubules.

What does the microfilament diagram illustrate?
Diagram of an actin filament, composed of two intertwined strands of actin subunits.

What does the intermediate filament diagram show?
Diagram of keratin proteins coiled together to form a fibrous subunit of intermediate filaments.

What structure is involved in the cytoskeleton?
What are microtubules made of?
Tubulin Polymers
What are microfilaments mainly composed of?
Actin Filaments
What do you call the fibers that help maintain cell shape?
Intermediate Filaments
What are plasmodesmata?
Channels connecting plant cells, allowing transfer of compounds.
What do gap junctions do?
Allow passage of ions/molecules between animal cells.
What is the function of tight junctions?
Create seals between cells to prevent extracellular fluid movement.
What do desmosomes do?
Fasten cells together using intermediate filaments.
What structure is labeled in this diagram? 
Plasmodesmata, allowing cell-to-cell communication.
What is the purpose of the tight junctions indicated in this diagram? 
Prevent intracellular fluid movement.
Which organs would dysfunction due to abnormal microtubules?
Which choice includes areas of contraction affected by microtubule dysfunction?
What tissues have projections that might be affected by abnormal microtubules?
Which ducts may experience dysfunction due to abnormal microtubules?
Which cells showing movement may be affected by abnormal microtubules?
What type of cells exhibit amoeboid movement that may be impacted?
What is the primary function of hepatocytes?
Drug detoxification, lipid synthesis/export, protein secretion.
What sub-cellular structures do hepatocytes have?
How many mitochondria do hepatocytes contain?
1000-2000 mitochondria per cell.
What happens to hepatocytes under chronic exposure to toxins?
Proliferation of smooth ER and increased drug tolerance.
What does the smooth ER in hepatocytes assist with?
What does the rough ER in hepatocytes mainly function for?
Protein secretion.
Show an image of hepatocyte ER structures.

Do erythrocytes (red blood cells) have a nucleus?
No, they do not have a nucleus in mammals.
What type of energy production do erythrocytes rely on?
ATP is produced from fermentation, as they lack mitochondria.
What cellular structures are absent in erythrocytes?
Why can't erythrocytes be targeted by viruses?
They lack DNA and RNA, preventing protein synthesis.
What is important for erythrocyte deformability?
The actin cytoskeleton and fluid cell membrane are crucial.
How does the blood type get determined?
Blood type is determined by the presence of glycoproteins.
What are the types of blood glycoproteins?
What is shown in this electron micrograph?
An erythrocyte (red blood cell). 
What is illustrated in the diagram of blood cells?
Different types of blood cells: O, A, B, AB. 
What are keratinocytes?
Cells that form the epidermis of the skin.
What happens to keratinocytes as they mature?
They lose their nucleus and other organelles.
What provides rigidity to keratinocytes?
An extensive network of intermediate filaments (keratins).
What role do desmosomes play in keratinocytes?
They provide adhesion and help in wound healing.
Do keratinocytes continue to divide?
They permanently withdraw from the cell cycle.
What is shown in the skin layers diagram?
The surface, upper keratinocytes, melanocytes, and basal keratinocytes.
What is the structure composition of skeletal muscle cells?
What is the basic contractile unit of muscle?
The sarcomere
What connects the sarcomere to other organelles?
Intermediate filaments
What is the role of sarcoplasmic reticulum in muscle cells?
Stores calcium, important for contraction
What condition is related to calcium leak in muscle cells?
Rigor mortis
What do skeletal muscle cells require to produce energy?
Lots of ATP from mitochondria
What is the role of actin and myosin?
Required for contraction of muscle cells
What do you observe in light micrograph of muscle cells?
Striated appearance
| WEEK 2 | LECTURE MATERIAL |
|---|---|
| Sep 8 - 14 | Week 2 Overview |
DSMs 6-7 * Due: Saturday Sep 20 at 11:59 PM * Grace period: Ends Tuesday Sep 23 at 11:59 PM
Lecture Quiz 1 * Opens: Saturday Sep 13 at 12:01 AM * Due: Monday Sep 15 at 11:59 PM * Grace period: Ends Thursday Sep 18 at 11:59 PM
Lab 1A - Online Pre-Lab * Opens: Monday Sep 8 at 12:01 AM * Quiz Due: Thursday Sep 11 at 11:59 PM * Grace period: Ends Sunday Sep 14 at 11:59 PM
Stuff You Should Know * Due: Thursday Sep 25 at 11:59 PM * Grace period: Ends Sunday Sep 28 at 11:59 PM
Note: No in-person lab this week; labs start in Week 3.
Light microscopy: Uses light beam through specimen.
Electron microscopy: Uses beam of electrons.

Phagocytosis: Macrophage digesting bacteria.

Cell Nucleus and Mitochondria: Microscopic view.

Definition: Cells with DNA in a nucleus. - Cytoplasm: Contains organelles in cytosol.
| Property | Animal Cell | Plant Cell |
|---|---|---|
| Cell Wall | Absent | Present (cellulose) |
| Vacuole | Small vacuoles | Large central vacuole |
| Centrioles | Present | Lower plant forms only |
| Chloroplast | Absent | Present |
| Lysosomes | Present | Usually absent |


Components: - Contains chromosomes made of chromatin (DNA + proteins). - Site of mRNA synthesis and export.
Nucleolus: - Site of ribosomal RNA transcription.

Definition: Complexes of protein and rRNA for protein synthesis. - Free Ribosomes: In cytosol. - Bound Ribosomes: On endoplasmic reticulum.

Components: - Nucleus, ER, Golgi apparatus, lysosomes. - Physically connected or via vesicles.

Description: Dynamic fiber network providing support and motility. - Components: Microtubules, microfilaments, intermediate filaments.

Types: - Plasmodesmata: Plant cell channels. - Gap Junctions: Enable communication in animal cells. - Tight Junctions: Seals preventing fluid passage. - Desmosomes: Anchor cells together.

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