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Flashcards in this deck (148)

Recherche en cours...
  • What is the focus of Molecular and Cell Biology?


    Study of cells, their structures, functions, and interactions.

    biology molecular_biology
  • What university offers BIOL 102?


    Queen's University

    biology education
  • Which week is referred to in the lecture title?


    Week 2

    lecture biology
  • What is the logo of Queen's University used for?


    Visual representation of the university.

    branding university
  • What is the due date for DSMs 6-7?


    Due Saturday Sep 20 at 11:59 PM. Grace period ends Tuesday Sep 23 at 11:59 PM.

    deadlines assessments
  • When does Lecture Quiz 1 open?


    Opens Saturday Sep 13 at 12:01 AM.

    deadlines quizzes
  • What is the due date for Lecture Quiz 1?


    Due Monday Sep 15 at 11:59 PM. Grace period ends Thursday Sep 18 at 11:59 PM.

    deadlines quizzes
  • When does Lab 1A - Background Research quiz open?


    Opens Monday Sep 8 at 12:01 AM.

    deadlines labs
  • What is the due date for Lab 1A quiz?


    Due Thursday Sep 11 at 11:59 PM. Grace period ends Sunday Sep 14 at 11:59 PM.

    deadlines labs
  • When is the due date for Stuff You Should Know activity?


    Due Thursday Sep 25 at 11:59 PM. Grace period ends Sunday Sep 28 at 11:59 PM.

    deadlines activities
  • When do in-person labs start?


    In-person labs don’t start until Week 3.

    labs schedule
  • What does the schedule include?


    It includes lecture material, quizzes, and assignment deadlines. schedule

    schedule lectures
  • What is light microscopy based on?


    Passing a beam of light through a specimen

    microscopy light
  • What does electron microscopy use?


    A beam of electrons over or through a specimen

    microscopy electron
  • Define resolution in microscopy.


    Minimum distance two points can be separated and still distinguishable

    microscopy resolution
  • What is the formula for magnification?


    Ratio of an object's image size to its real size

    microscopy magnification
  • What does contrast refer to?


    Difference in brightness between light and dark areas

    microscopy contrast
  • What does this diagram illustrate?


    Scales of biological entities and microscopy ranges Diagram illustrating scales of biological entities

    microscopy diagram
  • What is the process of phagocytosis? Phagocytosis


    Macrophage digesting bacteria.

    biology cellular_processes
  • What structures are shown in the microscopic view? Nucleus and Mitochondria


    • Nucleus (blue)
    • Mitochondria (yellow)
    biology cell_structure
  • Where in the cell would a culture of rapidly growing animal cells have the greatest concentration of radioactivity when adding non-toxic radioactive dTTP?


    • A. Nucleus
    • B. Nucleoid
    • C. Endoplasmic reticulum
    • D. Cytoplasm
    • E. Ribosomes
    cell_biology radioactivity nucleus
  • Where is the DNA found in eukaryotic cells?


    In the nucleus

    biology cell_structure
  • Eukaryotic cells are subdivided into which two types?


    • Animal cells
    • Plant cells
    biology cell_types
  • What is the interior substance of a cell called?


    The cytoplasm

    biology cell_structure
  • What jelly-like substance is found in the cytoplasm?


    The cytosol

    biology cell_structure
  • What is present in plant cells but absent in animal cells?


    • Cell wall
    • Chloroplasts
    • Plastids
    biology cell_comparison
  • What is unique about the vacuole in plant cells?


    One large central vacuole

    biology cell_comparison
  • Which structure is common to all animal cells?


    Presence of centrioles

    biology cell_structure
  • What organelle is responsible for energy production in both cell types?


    Mitochondrion

    biology cell_structure
  • Which organelle performs photosynthesis in plant cells?


    Chloroplast

    biology cell_structure
  • In which type of cell are lysosomes mostly found?


    Animal cells

    biology cell_structure
  • Which structure is absent in most plant cells?


    Cilia

    biology cell_structure
  • What structure supports the plant cell and is made of cellulose?


    Cell wall

    biology cell_structure
  • What diagram shows the organelles in a typical animal cell?


    Diagram of an animal cell with organelles labeled

    biology cell_diagram
  • What diagram shows the organelles in a typical plant cell?


    Diagram of a plant cell with organelles labeled

    biology cell_diagram
  • What do most plant cells look like?


    They resemble the standard textbook image of cells.

    biology plant_cells
  • What is a distinguishing feature of plant cells?


    They have visible cell walls.

    biology plant_cells
  • What can be seen clearly in most plant cells?


    Visible nuclei within the cells.

    biology plant_cells
  • What is shown in the microscopic image of onion cells?


    Rows of onion cells with distinct cell walls and nuclei. Micrograph of purple onion epidermal cells showing distinct cell walls and visible nuclei.

    biology microscopy onion_cells
  • What is the shape of epidermal pavement cells?


    These cells have an interlocking, irregular shape.

    biology plant_cells
  • What organelles are labeled in the epidermal pavement cell diagram?


    • Nucleus
    • Chloroplast
    • Vacuole
    • Plasma membrane
    • Peroxisome
    biology organelles
  • How do epidermal pavement cells contribute to plants?


    They provide protection and add strength & stability.

    biology plant_cells
  • Describe the appearance of green fluorescent plant epidermal cells.


    They have a characteristic jigsaw puzzle shape.

    biology plant_cells
  • What are ribosomes composed of?


    • Protein
    • rRNA
    biology ribosomes
  • What is the primary role of ribosomes?


    Protein synthesis

    biology cell
  • Where are free ribosomes located?


    Cytosol

    biology cell
  • What are bound ribosomes associated with?


    • Endoplasmic reticulum
    • Nuclear envelope
    biology cell
  • What does a ribosome consist of?


    • Large subunit
    • Small subunit
    biology ribosomes
  • What is the structure of a ribosome?


    3D model with complex structure.

    biology ribosomes
  • What are the components of the endomembrane system?


    • Nucleus
    • Endoplasmic reticulum
    • Golgi apparatus
    • Lysosomes
    • Vacuoles
    • Plasma membrane
    biology cell_structure
  • How are the components of the endomembrane system connected?


    Through direct physical continuity or the transfer of vesicles.

    biology cell_structure
  • What does the diagram of the endomembrane system include?


    • Nucleus
    • Rough ER
    • Smooth ER
    • Golgi apparatus
    biology diagrams
  • What does the nucleus contain?


    • Chromosomes
    • Chromatin (DNA + proteins)
    cell_biology nucleus
  • What is the function of the nucleus?


    • mRNA synthesis
    • Export
    cell_biology nucleus
  • What is the nuclear envelope?


    • A double membrane
    • Each membrane is a lipid bilayer
    cell_biology nucleus
  • What provides the stability to the nuclear envelope?


    • Nuclear lamina (protein filaments)
    cell_biology nucleus
  • What occurs in the nucleolus?


    • Transcription of ribosomal RNA (rRNA)
    • Ribosome assembly
    cell_biology nucleus
  • What accompanies the structure of the nucleus in diagrams?


    Diagram of the nucleus, showing the nuclear envelope, chromatin, nucleolus, and nuclear pores. Includes a close-up of the nuclear envelope structure.

    cell_biology nucleus
  • What does the label in the nuclear envelope diagram indicate?


    A diagram of the nuclear envelope showing labels such as nucleolus, DNA, and nuclear pores.

    cell_biology nucleus
  • What is the main function of the Smooth ER?


    • Lipid synthesis
    • Drug detoxification
    • Calcium storage
    biology cell
  • What does the Rough ER synthesize proteins for?


    • Exporting proteins
    biology cell
  • What processes is the Rough ER involved in?


    • Protein glycosylation
    • Membrane synthesis
    biology cell
  • What does the diagram illustrate?


    The structure of the rough and smooth endoplasmic reticulum

    biology cell
  • What is the function of the Golgi apparatus?


    • Sorts molecules
    • Releases vesicles for transport
    cell_biology organelles
  • What does the Golgi apparatus alter?


    • Structure of macromolecules
    cell_biology macromolecules
  • What does the Golgi apparatus synthesize?


    • Secreted polysaccharides
    cell_biology synthesis
  • What are the two faces of the Golgi apparatus?


    • Cis face (receiving)
    • Trans face (shipping)
    cell_biology golgi_apparatus
  • What are lysosomes?


    Membranous sacs with an acidic interior containing hydrolytic enzymes for digestion.

    cell_biology lysosomes
  • What is the function of hydrolytic enzymes in lysosomes?


    They are used for the hydrolysis of macromolecules.

    cell_biology enzymes
  • What process involves the fusion of a food vacuole with a lysosome?


    This process is called phagocytosis.

    cell_biology phagocytosis
  • What is autophagy?


    A process in which a vesicle containing cellular debris fuses with a lysosome for digestion.

    cell_biology autophagy
  • What are vacuoles?


    Large vesicles with an internal solution differing in composition from the cytoplasm.

    biology cell_structure
  • What is the function of the central vacuole?


    Important for maintaining cell structure.

    biology cell_structure
  • What does the central vacuole contain?


    • Ions
    • Toxins
    biology cell_structure
  • Where is the central vacuole found?


    In plant cells.

    biology cell_structure
  • What is shown in the electron micrograph related to vacuoles?


    The structure of the central vacuole within a plant cell.

    biology cell_structure
  • What are the primary sites of cellular respiration?


    Mitochondria produce ATP from fuels and oxygen.

    biology cellular_respiration
  • What are the main functions of chloroplasts?


    Sites of photosynthesis, producing sugars from carbon dioxide, water, and light.

    biology photosynthesis
  • What genetic material do mitochondria and chloroplasts contain?


    Both contain their own DNA and ribosomes.

    biology genetics
  • How do the ribosomes of mitochondria and chloroplasts compare?


    They are more related to bacteria than to eukaryotic versions.

    biology ribosomes
  • What does the Endosymbiont Theory describe?


    The evolutionary origins of mitochondria and chloroplasts.

    biology theory
  • What are the components involved in the Endosymbiont Theory?


    • Asgard archaeon
    • alphaproteobacterium
    • cyanobacterium
    biology evolution
  • What is illustrated in the diagram of the Endosymbiont Theory?


    An ancestral eukaryotic cell engulfing a nonphotosynthetic prokaryote to become a mitochondrion, and a photosynthetic prokaryote to become a chloroplast. Diagram illustrating the Endosymbiont Theory

    biology visual
  • Do alternative theories to the Endosymbiont Theory exist?


    Yes, alternate theories exist.

    biology theory
  • A mutation affecting polysaccharide modifications to proteins would likely disrupt which cellular structures?


    • Golgi apparatus
    • Extracellular matrix
    cell_biology mutations
  • What are the possible structures affected by the mutation?


    • Nuclear lamina
    • Nuclear matrix
    • Mitochondria
    • Nuclear pores
    • Secretory vesicles
    cell_biology structures
  • What is the cytoskeleton?


    A dynamic network of fibers extending throughout the cytoplasm.

    biology cell_structure
  • What are the three main components of the cytoskeleton?


    • Microtubules
    • Microfilaments (actin filaments)
    • Intermediate filaments
    biology cell_structure
  • What does the cytoskeleton provide for the cell?


    • Mechanical support
    • Structure
    • Anchorage for organelles
    biology cell_structure
  • What role does the cytoskeleton play in cell motility?


    Involved in the movement of the cell location and the movement of cell parts.

    biology cell_motility
  • What colors represent the components of the cytoskeleton in the micrograph?


    • Microtubules: Green
    • Microfilaments: Red
    • Nucleus: Blue
    biology cell_structure microscopy
  • What are the main components of the cytoskeleton?


    • Microtubules
    • Microfilaments
    • Intermediate Filaments
    biology cytoskeleton
  • What is the structure of Microtubules?


    • Hollow tubes
    • Diameter: 25 nm
    biology structure
  • What are the protein subunits of Microtubules?


    • Tubulin (α-tubulin and β-tubulin)
    biology proteins
  • What is the main function of Microtubules?


    • Maintenance of cell shape
    • Cell motility
    • Chromosome movements in cell division
    biology functions
  • What is the structure of Microfilaments?


    • Two intertwined strands of actin
    • Diameter: 7 nm
    biology structure
  • What are the main functions of Microfilaments?


    • Maintenance of cell shape
    • Muscle contraction
    • Cytoplasmic streaming
    biology functions
  • What is the structure of Intermediate Filaments?


    • Fibrous proteins coiled into cables
    • Diameter: 8-12 nm
    biology structure
  • What is the main function of Intermediate Filaments?


    • Anchorage of nucleus
    • Form nuclear lamina
    biology functions
  • What does the microtubule diagram represent?


    Diagram of a column of tubulin dimers, structural unit of microtubules. Microtubule structure

    biology diagrams
  • What does the microfilament diagram illustrate?


    Diagram of an actin filament, composed of two intertwined strands of actin subunits. Microfilament structure

    biology diagrams
  • What does the intermediate filament diagram show?


    Diagram of keratin proteins coiled together to form a fibrous subunit of intermediate filaments. Intermediate filament structure

    biology diagrams
  • What structure is involved in the cytoskeleton?


    • Microtubules
    • Microfilaments
    • Intermediate Filaments
    biology cytoskeleton
  • What are microtubules made of?


    Tubulin Polymers

    biology cell_structure
  • What are microfilaments mainly composed of?


    Actin Filaments

    biology cell_structure
  • What do you call the fibers that help maintain cell shape?


    Intermediate Filaments

    biology cell_structure
  • What are plasmodesmata?


    Channels connecting plant cells, allowing transfer of compounds.

    cell_biology plant
  • What do gap junctions do?


    Allow passage of ions/molecules between animal cells.

    cell_biology animal
  • What is the function of tight junctions?


    Create seals between cells to prevent extracellular fluid movement.

    cell_biology animal
  • What do desmosomes do?


    Fasten cells together using intermediate filaments.

    cell_biology adhesion
  • What structure is labeled in this diagram? Plant cell junctions


    Plasmodesmata, allowing cell-to-cell communication.

    cell_biology plant
  • What is the purpose of the tight junctions indicated in this diagram? Animal cell junctions


    Prevent intracellular fluid movement.

    cell_biology animal
  • Which organs would dysfunction due to abnormal microtubules?


    • Limbs and heart: areas with contraction
    • Microvilli, alveoli, glomeruli: increase surface area
    • All ducts: transport fluids
    • Sperm, larynx, trachea: contain flagella or cilia
    • Phagocytic cells: show amoeboid movement
    biology microtubules dysfunction
  • Which choice includes areas of contraction affected by microtubule dysfunction?


    • Limbs
    • Heart
    biology microtubules contraction
  • What tissues have projections that might be affected by abnormal microtubules?


    • Microvilli
    • Alveoli
    • Glomeruli
    biology microtubules surface_area
  • Which ducts may experience dysfunction due to abnormal microtubules?


    • Salivary glands
    • Sebaceous glands
    biology microtubules ducts
  • Which cells showing movement may be affected by abnormal microtubules?


    • Sperm
    • Larynx
    • Trachea
    biology microtubules movement
  • What type of cells exhibit amoeboid movement that may be impacted?


    • Phagocytic cells
    • White blood cells
    biology microtubules amoeboid
  • What is the primary function of hepatocytes?


    Drug detoxification, lipid synthesis/export, protein secretion.

    biology hepatocytes
  • What sub-cellular structures do hepatocytes have?


    • Extensive smooth ER
    • Rough ER
    • Golgi apparatus
    biology cell_structure
  • How many mitochondria do hepatocytes contain?


    1000-2000 mitochondria per cell.

    biology mitochondria
  • What happens to hepatocytes under chronic exposure to toxins?


    Proliferation of smooth ER and increased drug tolerance.

    biology toxins
  • What does the smooth ER in hepatocytes assist with?


    • Drug detoxification
    • Lipid synthesis/export
    biology smooth_er
  • What does the rough ER in hepatocytes mainly function for?


    Protein secretion.

    biology rough_er
  • Show an image of hepatocyte ER structures.


    Electron micrograph showing the extensive network of smooth endoplasmic reticulum (Smooth ER) and rough endoplasmic reticulum (Rough ER) within a hepatocyte.

    biology microscopy
  • Do erythrocytes (red blood cells) have a nucleus?


    No, they do not have a nucleus in mammals.

    biology erythrocytes
  • What type of energy production do erythrocytes rely on?


    ATP is produced from fermentation, as they lack mitochondria.

    biology metabolism
  • What cellular structures are absent in erythrocytes?


    • DNA
    • RNA
    • Golgi apparatus
    • ER
    biology cell_structure
  • Why can't erythrocytes be targeted by viruses?


    They lack DNA and RNA, preventing protein synthesis.

    biology viruses
  • What is important for erythrocyte deformability?


    The actin cytoskeleton and fluid cell membrane are crucial.

    biology cell_structure
  • How does the blood type get determined?


    Blood type is determined by the presence of glycoproteins.

    biology blood_type
  • What are the types of blood glycoproteins?


    • O
    • A
    • B
    • AB
    biology blood_type
  • What is shown in this electron micrograph?


    An erythrocyte (red blood cell). Erythrocyte Electron Micrograph

    biology microscopy
  • What is illustrated in the diagram of blood cells?


    Different types of blood cells: O, A, B, AB. Blood Type Glycoproteins

    biology blood_type
  • What are keratinocytes?


    Cells that form the epidermis of the skin.

    biology skin cells
  • What happens to keratinocytes as they mature?


    They lose their nucleus and other organelles.

    biology skin cells
  • What provides rigidity to keratinocytes?


    An extensive network of intermediate filaments (keratins).

    biology skin
  • What role do desmosomes play in keratinocytes?


    They provide adhesion and help in wound healing.

    biology skin
  • Do keratinocytes continue to divide?


    They permanently withdraw from the cell cycle.

    biology cells
  • What is shown in the skin layers diagram?


    The surface, upper keratinocytes, melanocytes, and basal keratinocytes.

    biology anatomy
  • What is the structure composition of skeletal muscle cells?


    • Multinucleated
    • Lots of mitochondria
    • Actin and myosin for contraction
    biology muscle_cells
  • What is the basic contractile unit of muscle?


    The sarcomere

    biology muscle_cells
  • What connects the sarcomere to other organelles?


    Intermediate filaments

    biology muscle_cells
  • What is the role of sarcoplasmic reticulum in muscle cells?


    Stores calcium, important for contraction

    biology muscle_cells
  • What condition is related to calcium leak in muscle cells?


    Rigor mortis

    biology muscle_cells
  • What do skeletal muscle cells require to produce energy?


    Lots of ATP from mitochondria

    biology muscle_cells
  • What is the role of actin and myosin?


    Required for contraction of muscle cells

    biology muscle_cells
  • What do you observe in light micrograph of muscle cells?


    Striated appearance

    biology muscle_cells
Notes de cours

Upcoming Deadlines

Schedule

WEEK 2 LECTURE MATERIAL
Sep 8 - 14 Week 2 Overview

Assessments and Activities

DSMs 6-7 * Due: Saturday Sep 20 at 11:59 PM * Grace period: Ends Tuesday Sep 23 at 11:59 PM

Lecture Quiz 1 * Opens: Saturday Sep 13 at 12:01 AM * Due: Monday Sep 15 at 11:59 PM * Grace period: Ends Thursday Sep 18 at 11:59 PM

Lab 1A - Online Pre-Lab * Opens: Monday Sep 8 at 12:01 AM * Quiz Due: Thursday Sep 11 at 11:59 PM * Grace period: Ends Sunday Sep 14 at 11:59 PM

Stuff You Should Know * Due: Thursday Sep 25 at 11:59 PM * Grace period: Ends Sunday Sep 28 at 11:59 PM

Note: No in-person lab this week; labs start in Week 3.

Principles of Microscopy

Light microscopy: Uses light beam through specimen.

Electron microscopy: Uses beam of electrons.

Key Concepts

  • Resolution: Minimum distance to distinguish two points.
  • Magnification: Image size to real size ratio.
  • Contrast: Brightness difference between areas.

Diagram illustrating microscopy scales

Microscopy Examples

Notable Examples

  • Phagocytosis: Macrophage digesting bacteria. Phagocytosis involving macrophage

  • Cell Nucleus and Mitochondria: Microscopic view. Cell nucleus and mitochondria

Eukaryotic Cells

Definition: Cells with DNA in a nucleus. - Cytoplasm: Contains organelles in cytosol.

Cell Comparison

Property Animal Cell Plant Cell
Cell Wall Absent Present (cellulose)
Vacuole Small vacuoles Large central vacuole
Centrioles Present Lower plant forms only
Chloroplast Absent Present
Lysosomes Present Usually absent

Animal cell diagram
Plant cell diagram

Nucleus

Components: - Contains chromosomes made of chromatin (DNA + proteins). - Site of mRNA synthesis and export.

Nuclear Envelope

  • Double membrane with nuclear lamina for structure.

Nucleolus: - Site of ribosomal RNA transcription.

Diagram of the nucleus

Ribosomes

Definition: Complexes of protein and rRNA for protein synthesis. - Free Ribosomes: In cytosol. - Bound Ribosomes: On endoplasmic reticulum.

Ribosome structure

Endomembrane System

Components: - Nucleus, ER, Golgi apparatus, lysosomes. - Physically connected or via vesicles.

Endomembrane system diagram

Cytoskeleton

Description: Dynamic fiber network providing support and motility. - Components: Microtubules, microfilaments, intermediate filaments.

Functions:

  • Cell shape and movement.

Cytoskeleton micrograph

Cell Junctions

Types: - Plasmodesmata: Plant cell channels. - Gap Junctions: Enable communication in animal cells. - Tight Junctions: Seals preventing fluid passage. - Desmosomes: Anchor cells together.

Cell junctions illustration